Reverse Transcription Basics
Reverse Transcription Basics The process of creating DNA from an RNA template is known as reverse transcription. Reverse transcriptases, or RNA-dependent DNA polymerases, are the enzymes that propel this process. Reverse transcriptases are found in retroviruses and prokaryotic and eukaryotic organisms in their natural states. When a viral particle enters a target cell's cytoplasm, reverse transcription starts. As a component of an as-yet-uncharacterized nucleoprotein complex, the viral RNA genome penetrates the cytoplasm. Through a complex set of steps, reverse transcription creates a linear DNA duplex in the cytoplasm. Although the DNA and its RNA template are colinear, the DNA has terminal duplications called long terminal repeats (LTRs) that are absent from viral RNA. Current reverse transcription models suggest that in order to produce the LTRs, two specific template switches called "jumps" or strand-transfer reactions are needed. The two unique enzymatic activities...